How to export file to tiff from zen 2.3 lite

HOW TO EXPORT FILE TO TIFF FROM ZEN 2.3 LITE PRO
HOW TO EXPORT FILE TO TIFF FROM ZEN 2.3 LITE SOFTWARE

Save images in the OME-TIFF format, not TIFF. Images acquired with the Hamamatsu camera (fluorescence) Instructions and links to macros to do this can be found here. For best results, flatfield your brightfield images.

HOW TO EXPORT FILE TO TIFF FROM ZEN 2.3 LITE SOFTWARE

You must do this each time you save a subset of files, as there is a bug in the software that results in an incorrectly saved image if you donРІР‚в„ўt do this every time, even if the save options have not been modified. Make sure you click on the РІР‚СљOptionsРІР‚В¦РІР‚Сњ button and ensure РІР‚СљConvert to RGB for publicationРІР‚Сњ is clicked and РІР‚СљScaleРІР‚Сњ is unclicked.

Images acquired with the Retiga camera (color) For more information on what settings you should keep track of, contact the MSL staff or read our guidelines. Volocity does not save image metadata, so you will need to keep your own record of the conditions you used to acquire data (exposure, gain, offset, binning, channels, ND filters on BX61, LED power on IX81). czi file, it is formatted in a way that makes it cumbersome to read. This retains the metadata about how the image was acquired, lets you reuse the settings, is well read by our deconvolution software (Autoquant), can be opened in FIJI, and its metadata can be read properly by the free version of ZeissРІР‚в„ў software (Zen lite black) in Windows 10. Specific recommendations for different software and scope combinations: Zeiss Zen (LSM700, LSM710): In addition to losing all the metadata detailing how you took your images you will also compress the data in a РІР‚СљlossyРІР‚Сњ manner, which means it will be irreversibly modified and degraded. This file format is fine for pictures of loved ones, cute animals and pretty sunsets, not for scientific data. This is very useful when you are trying to ensure the reproducibility of your experiments. Saving a file with metadata will allow you to automatically record all of those acquisition settings, and reuse them. Metadata is data about the data, which in this context typically means the acquisition settings. If there is a file format that saves metadata, use it.

HOW TO EXPORT FILE TO TIFF FROM ZEN 2.3 LITE PRO

2.5 Imspector Pro (UltraMicroscope II РІР‚вЂњ Snape):.2.3 Metamorph (IX81 РІР‚вЂњ Luna, IX70 РІР‚вЂњ Neville):.2.2.2 Images acquired with the Hamamatsu camera (fluorescence).2.2.1 Images acquired with the Retiga camera (color).2.2 Volocity (BX61 РІР‚вЂњ Neville, IX81 РІР‚вЂњ Luna).2 Specific recommendations for different software and scope combinations:.

How to export file to tiff from zen 2.3 lite